Journal: International Journal of Molecular Medicine

Article Title: Receptor FGFRL1 does not promote cell proliferation but induces cell adhesion

doi: 10.3892/ijmm.2016.2601

Figure Lengend Snippet: Kinexus antibody microarray. Fibroblast growth factor receptor-like protein 1 (FGFRL1) inducible cells (clone K3F) were cultivated for 32 h in the presence or absence of the inducer doxycycline. Protein extracts were prepared and analyzed on Kinexus antibody microarrays containing more than 650 different antibodies against various signaling proteins. No significant differences were observed between doxycycline-treated and untreated cells (not shown). To verify some of the results, the two protein extracts were separated on SDS polyacrylamide gels, transferred to an immunoblot membrane and probed with antibodies against 18 selected proteins as indicated using a multiscreen apparatus from BioRad. The immunoblot was analyzed with a chemiluminescence imager. No significant differences were observed between doxycycline-induced (lower panel) and uninduced (top panel) cells. The migration positions of molecular weight markers are shown in the left and right margins by short horizontal lines. These lines correspond (from top to bottom) to 250 K, 150 K, 100 K, 75 K, 50 K, 37 K, 25 K, 20 K and 15 K.

Article Snippet: The extracts were analyzed by the Kinexus antibody microarray service, which utilizes >650 verified antibodies against most well-studied signaling proteins, including phospholipase Cγ, PI3-kinase, STAT and Akt.

Techniques: Microarray, Western Blot, Migration, Molecular Weight